MALACHITE GREEN BROTH BASE 500GR MERCK
Modelo: 1103290500
-
R$2.205,82
ou até 3x de R$735,27 sem juros
Economize 5% no PIX: R$2.095,53
ou 3.5% no boleto: R$2.128,62
Descrição
MALACHITE GREEN BROTH BASE MERCK
RENDIMENTO 8,4 (SIMPLES) OU 25,1g/l (TRIPLO)
EMBALAGEM 500GR
Typical Composition (g/litre)
Peptone from meat 5.0; meat extract 3.0; di-potassium hydrogen phosphate 0.37.
These values are valid for the single-strength broth.
Preparation
Base Broth:
Suspend 8.4 g (single-strength broth) or 25.1 g (triple-strength broth) in 1 litre of demineralized water. Dispense in 50 ml volumes into suitable culture vessels and autoclave (15 min at 121°C). The broth base is clear and yellow-brown.
Malachite-green solution:
Suspend 0.15 g malachite-green oxalate in 90 ml of deminera-lized water and sterilize by filtration.
Complete broth:
Add to 50 ml of cooled base broth 0.3 ml (single-strength broth) or 0.9 ml (triple-strength broth) of the malachite-green solution under sterile conditions.
pH: 7.3 ± 0.2 at 25 °C.
The complete broth is clear and green.
Alternative method of preparation:
If preferred, the malachite-green solution may also be added before distributing the base broth into the vessels. In this case it has to be autoclaved first. Then add to 1 litre of base broth 6ml (single-strength broth) or 18 ml (triple-strength broth) of malachite-green solution under sterile conditions and dispense
in 50 ml volumes into sterile vessels.
RENDIMENTO 8,4 (SIMPLES) OU 25,1g/l (TRIPLO)
EMBALAGEM 500GR
Typical Composition (g/litre)
Peptone from meat 5.0; meat extract 3.0; di-potassium hydrogen phosphate 0.37.
These values are valid for the single-strength broth.
Preparation
Base Broth:
Suspend 8.4 g (single-strength broth) or 25.1 g (triple-strength broth) in 1 litre of demineralized water. Dispense in 50 ml volumes into suitable culture vessels and autoclave (15 min at 121°C). The broth base is clear and yellow-brown.
Malachite-green solution:
Suspend 0.15 g malachite-green oxalate in 90 ml of deminera-lized water and sterilize by filtration.
Complete broth:
Add to 50 ml of cooled base broth 0.3 ml (single-strength broth) or 0.9 ml (triple-strength broth) of the malachite-green solution under sterile conditions.
pH: 7.3 ± 0.2 at 25 °C.
The complete broth is clear and green.
Alternative method of preparation:
If preferred, the malachite-green solution may also be added before distributing the base broth into the vessels. In this case it has to be autoclaved first. Then add to 1 litre of base broth 6ml (single-strength broth) or 18 ml (triple-strength broth) of malachite-green solution under sterile conditions and dispense
in 50 ml volumes into sterile vessels.
