LACTOSE TTC AGAR COM TERGITOL 7 MERCK - EMB 500G
Modelo: 1076800500
-
R$964,50
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Economize 5% no PIX: R$916,28
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Descrição
LACTOSE TTC AGAR WITH TERGITOL 7 MERCK
RENDIMENTO 53,9g/l
EMBALAGEM 500G
Mode of Action
Degradation of lactose to acid is indicated by the pH indicator bromothymol blue, which changes the colour of the medium under the membrane to yellow. Selectivity is achieved by the use of sodium heptadecylsulfate (Tergitol®7) and 2,3,5-Triphenyltetrazoliumchloride (TTC) to inhibit most Grampositive bacteria. TTC is also part of the differential system. The reduction of TTC by lactose-negative bacteria produces dark red colonies. Lactose-positive E. coli and coliform bacteria reduce TTC weakly; hence their colonies are yellow-orange.
Typical Composition (g/litre)
Lactose 20.0; peptone 10.0; yeast extract 6.0; meat extract 5.0; bromothymol blue 0.05; Tergitol®7 0.1; agar-agar 12.7.
Additive: TTC 0.025.
Preparation
Suspend 53.9 g in 1 litre of demin. water, dissolve and autoclave (121 °C, 15 min). Cool the medium in a water bath to 45-50 °C, add 5 ml of a sterile filtrated 0.05 % aqueous solution of TTC to 100 ml basal medium. Mix homogeneously and pour the medium into Petridishes. The agar layer should have a height of at least 5 mm.
pH: 7.2 ± 0.2 at 25 °C.
The prepared medium is clear and green.
The TTC-solution and the medium is stable for 4 week when stored at +2° - +8°C and protected from light.
RENDIMENTO 53,9g/l
EMBALAGEM 500G
Mode of Action
Degradation of lactose to acid is indicated by the pH indicator bromothymol blue, which changes the colour of the medium under the membrane to yellow. Selectivity is achieved by the use of sodium heptadecylsulfate (Tergitol®7) and 2,3,5-Triphenyltetrazoliumchloride (TTC) to inhibit most Grampositive bacteria. TTC is also part of the differential system. The reduction of TTC by lactose-negative bacteria produces dark red colonies. Lactose-positive E. coli and coliform bacteria reduce TTC weakly; hence their colonies are yellow-orange.
Typical Composition (g/litre)
Lactose 20.0; peptone 10.0; yeast extract 6.0; meat extract 5.0; bromothymol blue 0.05; Tergitol®7 0.1; agar-agar 12.7.
Additive: TTC 0.025.
Preparation
Suspend 53.9 g in 1 litre of demin. water, dissolve and autoclave (121 °C, 15 min). Cool the medium in a water bath to 45-50 °C, add 5 ml of a sterile filtrated 0.05 % aqueous solution of TTC to 100 ml basal medium. Mix homogeneously and pour the medium into Petridishes. The agar layer should have a height of at least 5 mm.
pH: 7.2 ± 0.2 at 25 °C.
The prepared medium is clear and green.
The TTC-solution and the medium is stable for 4 week when stored at +2° - +8°C and protected from light.
